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Image Search Results
Journal: Cell reports
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
doi: 10.1016/j.celrep.2023.113581
Figure Lengend Snippet: (A and B) HEK293T cells stably expressing ΔCARD Dmrb-CASP11 (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Article Snippet: Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), FLAG M2 monoclonal Ab (Sigma, F3165), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), CASP1 p20 Rabbit polyclonal Ab (Cell Signaling Tech, 2225s), CASP1 p12/10 Rabbit monoclonal Ab (Abcam, ab179515), CASP4 Rabbit polyclonal Ab (Cell Signaling Tech, 4450S), CASP5 Rabbit monoclonal Ab (Cell Signaling Tech, 46680S), Myc Mouse monoclonal Ab (Cell Signaling Tech, 2276S), V5 Rabbit monoclonal Ab (Cell Signaling Tech, 13202S), hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech, 200-01A), PARP Rabbit polyclonal Ab (Cell Signaling Tech, 9542S), HA Rabbit monoclonal Ab (Cell Signaling Tech, 3724S), mIL-1β Goat polyclonal Ab (R&D systems, AF-401-NA),
Techniques: Stable Transfection, Expressing, Transfection, Construct, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Incubation, Activity Assay, Recombinant
Journal: Cell reports
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
doi: 10.1016/j.celrep.2023.113581
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), FLAG M2 monoclonal Ab (Sigma, F3165), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), CASP1 p20 Rabbit polyclonal Ab (Cell Signaling Tech, 2225s), CASP1 p12/10 Rabbit monoclonal Ab (Abcam, ab179515), CASP4 Rabbit polyclonal Ab (Cell Signaling Tech, 4450S), CASP5 Rabbit monoclonal Ab (Cell Signaling Tech, 46680S), Myc Mouse monoclonal Ab (Cell Signaling Tech, 2276S), V5 Rabbit monoclonal Ab (Cell Signaling Tech, 13202S), hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech, 200-01A), PARP Rabbit polyclonal Ab (Cell Signaling Tech, 9542S), HA Rabbit monoclonal Ab (Cell Signaling Tech, 3724S), mIL-1β Goat polyclonal Ab (R&D systems, AF-401-NA),
Techniques: Recombinant, Virus, CyQUANT Assay, LDH Cytotoxicity Assay, DC Protein Assay, Enzyme-linked Immunosorbent Assay, Software