caspase 11 Search Results


93
R&D Systems polyclonal rabbit anti mouse caspase 11
Polyclonal Rabbit Anti Mouse Caspase 11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit anti mouse caspase 11 - by Bioz Stars, 2026-03
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94
Novus Biologicals casp11 rat monoclonal ab
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Casp11 Rat Monoclonal Ab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/casp11 rat monoclonal ab/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
casp11 rat monoclonal ab - by Bioz Stars, 2026-03
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93
ProSci Incorporated anti card11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Anti Card11, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti card11/product/ProSci Incorporated
Average 93 stars, based on 1 article reviews
anti card11 - by Bioz Stars, 2026-03
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94
Santa Cruz Biotechnology caspase 11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Caspase 11, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 11/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
caspase 11 - by Bioz Stars, 2026-03
94/100 stars
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95
Cell Signaling Technology Inc rat monoclonal anti mouse caspase 11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Rat Monoclonal Anti Mouse Caspase 11, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat monoclonal anti mouse caspase 11/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rat monoclonal anti mouse caspase 11 - by Bioz Stars, 2026-03
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90
ProSci Incorporated rabbit anti carma1
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Rabbit Anti Carma1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti carma1/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
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95
Novus Biologicals caspase 11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Caspase 11, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 11/product/Novus Biologicals
Average 95 stars, based on 1 article reviews
caspase 11 - by Bioz Stars, 2026-03
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94
R&D Systems mab8648
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Mab8648, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab8648/product/R&D Systems
Average 94 stars, based on 1 article reviews
mab8648 - by Bioz Stars, 2026-03
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94
Novus Biologicals anti casp11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Anti Casp11, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti casp11/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
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94
R&D Systems caspase 11
(A and B) HEK293T cells stably expressing ΔCARD <t>Dmrb-CASP11</t> (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.
Caspase 11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 11/product/R&D Systems
Average 94 stars, based on 1 article reviews
caspase 11 - by Bioz Stars, 2026-03
94/100 stars
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Image Search Results


(A and B) HEK293T cells stably expressing ΔCARD Dmrb-CASP11 (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.

Journal: Cell reports

Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases

doi: 10.1016/j.celrep.2023.113581

Figure Lengend Snippet: (A and B) HEK293T cells stably expressing ΔCARD Dmrb-CASP11 (A) or 2xFLAG-CASP11 (B) were transiently transfected with the indicated constructs. After 24 h, samples were treated with AP20187 (1 μM) (A) or LPS (25 μg/mL) (B) for 24 h and then analyzed for LDH release and immunoblotting. (C and E) Raw 264.7 macrophages were primed with LPS (5 μg/mL) for 4 h and then transfected with LPS (25 μg/mL) for 6 h before LDH release and immunoblot analysis (C) and IL-1 receptor activation by IL-1β (E). (D) Raw 264.7 macrophages were treated as in (C) and then analyzed for IL-1β release by ELISA. (F) HEK293T cell lysates expressing either human IL-18 or mouse IL-18 were incubated with one activity unit of the indicated recombinant caspases for 1 h or 24 h and then analyzed by immunoblotting. Data are means ± SEM of three biological replicates. ***p < 0.001, **p < 0.01, and *p < 0.05 by two-sided Student’s t test. Data are representative of three or more independent experiments. * = non-specific bands. The small m or h represents mouse or human proteins, respectively.

Article Snippet: Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), FLAG M2 monoclonal Ab (Sigma, F3165), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), CASP1 p20 Rabbit polyclonal Ab (Cell Signaling Tech, 2225s), CASP1 p12/10 Rabbit monoclonal Ab (Abcam, ab179515), CASP4 Rabbit polyclonal Ab (Cell Signaling Tech, 4450S), CASP5 Rabbit monoclonal Ab (Cell Signaling Tech, 46680S), Myc Mouse monoclonal Ab (Cell Signaling Tech, 2276S), V5 Rabbit monoclonal Ab (Cell Signaling Tech, 13202S), hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech, 200-01A), PARP Rabbit polyclonal Ab (Cell Signaling Tech, 9542S), HA Rabbit monoclonal Ab (Cell Signaling Tech, 3724S), mIL-1β Goat polyclonal Ab (R&D systems, AF-401-NA), CASP11 Rat monoclonal Ab (Novus Biologicals, NB120-10454), FKBP12 Rabbit polyclonal Ab (Abcam, ab24373).

Techniques: Stable Transfection, Expressing, Transfection, Construct, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Incubation, Activity Assay, Recombinant

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases

doi: 10.1016/j.celrep.2023.113581

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), FLAG M2 monoclonal Ab (Sigma, F3165), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), CASP1 p20 Rabbit polyclonal Ab (Cell Signaling Tech, 2225s), CASP1 p12/10 Rabbit monoclonal Ab (Abcam, ab179515), CASP4 Rabbit polyclonal Ab (Cell Signaling Tech, 4450S), CASP5 Rabbit monoclonal Ab (Cell Signaling Tech, 46680S), Myc Mouse monoclonal Ab (Cell Signaling Tech, 2276S), V5 Rabbit monoclonal Ab (Cell Signaling Tech, 13202S), hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech, 200-01A), PARP Rabbit polyclonal Ab (Cell Signaling Tech, 9542S), HA Rabbit monoclonal Ab (Cell Signaling Tech, 3724S), mIL-1β Goat polyclonal Ab (R&D systems, AF-401-NA), CASP11 Rat monoclonal Ab (Novus Biologicals, NB120-10454), FKBP12 Rabbit polyclonal Ab (Abcam, ab24373).

Techniques: Recombinant, Virus, CyQUANT Assay, LDH Cytotoxicity Assay, DC Protein Assay, Enzyme-linked Immunosorbent Assay, Software